Due to the low numbers, only trends can be suggested from these data but we find the down-regulation of LAT gene expression to ~65% in 16p11.2 distal carriers (Supplementary Figure 7, Supplementary Table 11) particular interesting due to recent results in zebrafish: These showed that of the nine genes in 16p11.2 distal, only over-expression of LAT induced a decrease in cell proliferation in the brain with a concomitant microcephaly phenotype [44]. This evidence concerns the gene LAT and microcephaly.