Thus, this analysis confirmed the frequent mutations in glioblastomas of PTEN, TP53, EGFR, PI3KCA, PIK3R1, NF1, RB1, IDH1, and PDGFRA. Analysis of the mostly frequently mutated genes or with frequent copy number alterations in glioblastoma showed that various gene sets are very frequently altered in these tumors in a pattern of mutual exclusivity [16]. The gene discussed is RB1; the disease is glioblastoma.