To examine the inhibitory effect of the blocking peptides against the C-terminal tails of tetraspanins in a second viral infection model, EA.hy926, HEC-LTT (human endothelial cell-large T antigen and telomerase) and HFF (human foreskin fibroblast) cells were incubated with blocking peptides of the C-terminal tail of tetraspanins CD9, CD63, CD81, and CD151 or the control peptide and then exposed to the HCMV strain TB40/E. This evidence concerns the gene CD81 and viral infectious disease.