MYO1D and colitis: To further confirm that mutations in Myo1d result in a DSS-induced colitis susceptibility phenotype, CRISPR/Cas9-mediated targeting was used to generate a 96 bp insertion (96ins) in Myo1d, which resulted in the in-frame addition of 32 amino acids in the TH1 domain of the MYO1D protein (Fig. 1G,H).