CASP3 and cancer: Furthermore, the Caspase-3 activity was also observed after 6 h of treatment with R2PLx (Figure 6D), and was found similar to Annexin V-FITC/PI staining results, as well as the low LDH leakage at 6 h (Figure 6C), suggesting R2PLx-induced cancer cell death involved in the activation of intrinsic apoptotic pathway.