Using qRT-PCR analysis, no significant difference in DLC1 expression was observed in lymph node metastases compared with their matched primary breast tumors (n = 23 pairs; p = 0.2474 using Wilcoxon test of simple one-way ANOVA- GraphPad Prism 7.03;Fig 1D). These results suggest that loss of DLC1 expression may occur at the primary tumor stage rather than in metastatic lesions. The gene discussed is DLC1; the disease is breast neoplasm.