Since cryo-EM is too low throughput to study a large number of cases, we also used immuno-gold negative-stain electron microscopy (immuno-EM) to determine the morphologies and tau repeat composition of the core of PHFs and SFs from frontal cortex of the four cases used for cryo-EM, an additional 12 cases with the typical form of sporadic AD, and one more case with dominantly inherited disease. Here, MAPT is linked to Alzheimer disease.