23 cases were isocitrate dehydrogenase (IDH)- mutant WHO grade II diffuse astrocytomas, 15 cases were IDH-mutant WHO grade III anaplastic astrocytomas, 4 cases were of anaplastic oligodendroglioma (NOS), and 30 cases of IDH-wild type WHO grade IV glioblastoma. This evidence concerns the gene IDH3A and diffuse astrocytoma.