To examine the above hypothesis that METCAM/MUC18 may promote the malignant propensity of ovarian carcinoma [8], we used the above clones to test the impact of METCAM/MUC18 over-expression on tumorigenesis and malignant propensity of the cells in an athymic female nude mouse model via two injection routes, the (non-orthotopic) subcutaneous (SC) injection route and the (orthotopic) intraperitoneal cavity route [23]. This evidence concerns the gene MCAM and ovarian carcinoma.