HSPA5 and metabolic dysfunction-associated steatotic liver disease: To further investigate lipid accumulation, cells were stained with Nile Red to quantify lipid droplets within HepG2 cells after stimulation with HSP70, GRP78 and serum from obese IR, NAFLD subjects before and after metabolic surgery, and after HSP70/GRP78 immunodepletion (Fig. 3k).