To determine if Plin2 protein expression was increased in obese IR, NAFLD subjects, flow cytometry analysis was performed in monocyte subpopulations (CD14+CD16−, CD14++CD16+ and CD14+CD16+) and in total PBMCs of obese IR, NAFLD subjects and of healthy controls. Here, PLIN2 is linked to metabolic dysfunction-associated steatotic liver disease.