The importance of the NF‐κB signaling pathway in CRC has been proven by its frequent activation and association with rapid cancer progression once the expression of its transcriptional targets is upregulated.26 A previous study also suggested an essential role of NF‐κB signaling in the regulation of chemokines, such as CXCL1 and CXCL4.27 Our results demonstrated that the inhibition of IKKβ and the NF‐κB p65 subunit at either the gene or protein level significantly downregulated CXCL1, CXCL2, and CXCL4 expression in CRC cells. This evidence concerns the gene CXCL1 and colorectal carcinoma.