Based on the comet data and previously reported genetic association study of FEN1 with FECD in Polish population, we aimed to assess the genetic involvement of FEN1 gene in conferring insufficient DNA repair and genotyped two polymorphisms present in its regulatory untranslated regions, c.-69G>A (rs174538; 5’UTR) and c.4150G>T (rs4246215; 3’UTR) in 79 FECD cases and 234 control individuals in Indian population. This evidence concerns the gene FEN1 and Fuchs endothelial corneal dystrophy.