PIAS3 and Miyoshi myopathy: Initially, we investigated endogenous PIAS3 expression in MM cell lines; western blotting and qRT‐PCR were performed to detect PIAS3 protein and mRNA levels, respectively, in four MM cell lines (211H, H2052, H28, H2452); a NSCLC cell line (A549) was used as a positive control (Fig. 1).