To investigate the role of Runx3 in mediating group 1 ILCs and NCR+ILC3s against intracellular bacterial infection, we generated PLZF-cre Runx3 cKO mice (Figure S1A) (PLZF-cre Runx3 fl/fl are hereafter referred to as Runx3 cKO mice). Here, RUNX3 is linked to bacterial infectious disease.