In this study, we detected all currently known subsets of CD4+ T cells and their functional cytokine expression, and we found for the first time that Th1/IFN-γ, Th2/IL-4, Th9/IL-9, Th17/IL-17, and Th22/IL-22 were increased in AD patients and positively associated with the presence of AD, while Th2/IL-4 and Treg/IL-35 were reduced in AD patients and negatively related to the presence of AD. Here, CD4 is linked to Alzheimer disease.