Interestingly, increased levels of triple-positive (IFN-γ+TNF-α+IL-2+) and double-positive (IFN-γ+TNF-α+) antigen-specific CD4+CD44high T cells were observed in the BCG-, GrpE-immunised group compared to the control groups in the spleen and lung at 4 and 8 weeks post-infection (Fig. 4), and triple-positive (IFN-γ+TNF-α+IL-2+) and double-positive (IFN-γ+TNF-α+ and TNF-α+IL-2+) antigen-specific CD8+CD44high T cells were observed in the lung at 4 weeks post-infection. This evidence concerns the gene TNF and infection.