To validate whether the NKX2-1-AS1 transcript annotated in public databases (ENST00000521292.2, hg38 chr14:36,519,278–36,523,016, Fig. 1A) is detected in lung tumors, we measured the expression of NKX2-1-AS1 and NKX2-1 using qPCR in a select number of lung adenocarcinomas (AC, n = 8) and squamous cell carcinomas (SCC, n = 8) and their matched non-tumor controls, described in Table 1. This evidence concerns the gene LGR5 and neoplasm.