Moreover, the full-length TFPI-2, which interacts with both actini-4 and myosin-9 shows a stronger repression of cell migration and invasion than the TFPI-2/N + KD1 truncate that only interacts with myosin-9, the mutual interactions of TFPI-2 with myosin-9 or actinin-4 could lead to a combined effect in suppressing migration and invasion of breast cancer cells. The gene discussed is MYH9; the disease is breast cancer.