When we added fixed effects for the 4 MetS CpG sites into the model without shared environment-related variance components, two of the CpG sites (cg00574958 at CPT1A, cg06500161 at ABCG1; P value <7E-5) were strongly associated with MetS and another site (cg18181703 at SOCS3; P value = 0.07) was suggestively associated with MetS. The gene discussed is ABCG1; the disease is metabolic syndrome.