ddPCR analysis of splicing in urine cells confirmed an increase of MBNL2 exon 6 inclusion in DM1 vs. UA samples that was evident by RT-PCR, and revealed statistically significant differences in splicing of INSR, MBNL1, CLASP1, and MAP3K4 between the DM1 and UA groups that were non-significant by RT-PCR (Fig. 8). The gene discussed is MBNL2; the disease is myotonic dystrophy type 1.