Using values from INSR, MBNL2, MBNL1, CLASP1, and MAP3K4 and principal component analysis, we created a ddPCR-based composite splicing biomarker score for each specimen, demonstrating a wide separation between the symptomatic DM1 and UA groups, and an intermediate position of the asymptomatic group (Fig. 9b and Supplementary Table 7). This evidence concerns the gene INSR and myotonic dystrophy type 1.