Similar to the results obtained in mice, the percentage of the stained area of renal α-klotho was also reduced by nearly 2-fold in tubules of Hp 2-2 DN patients (7.5 ± 2.36%) compared with DM Hp 1-1 genotype (14.02 ± 5.1%, p < 0.05) or with CKD nonDM Hp 2-2 genotype (14.58 ± 5.34%, p < 0.05, Figures 4(a) and 4(b)). This evidence concerns the gene HP and chronic kidney disease.