TJP1 and obesity due to melanocortin 4 receptor deficiency: The expression of tight junction proteins like (mRNA and protein) occludin, zonulin-1 (Zo1), and junctional adhesion molecule (Jam), as well as mucin synthesis gene mRNAs (Muc2 and Muc6), was significantly decreased in Lepob/ob mice (Figures 1(b)–1(d)), suggesting that obesity resulting from leptin deficiency decreased expression of tight junction and mucin synthesis genes, which might result to leaky gut with increased permeability.