To molecularly dissect the role of NF-κB in TSCC, prospectively collected one hundred fresh tongue tissue biopsies comprising precancer (n = 20), cancer (n = 50) and adjacent normal controls (n = 30) and a HPV+ve (UPCI:SCC090) and a HPV−ve (AW13516) tongue cancer cell lines were analyzed. The gene discussed is NFKB1; the disease is tongue cancer.