Additionally, the OvaSure screening test (which uses six biomarkers: leptin, prolactin, osteopontin, insulin-like growth factor II, macrophage inhibitory factor, and CA-125) is no longer reliable because some of the markers are not expressed timely enough to be useful for early stage detection of ovarian cancer (Mai et al., 2011). The gene discussed is MUC16; the disease is ovarian cancer.