PTEN and neoplasm: DNA sequencing (whole exome and MCPyV) detected several tumor-associated/somatic mutations (e.g., PTEN) in the pre-treatment tumor, but no additional mutations explaining immune escape at acquired resistance, including no mutations or loss of-heterozygosity in the HLA-B gene, sequenced HLA-B promoter region, or targeted MCPyV epitope (Fig. 1d, Supplementary Data 1, Supplementary Table 2).