PGAM5 and hepatocellular carcinoma: To further asses and confirm whether PGAM5 promotes HCC oncogenic phenotypes, we stably knocked down PGAM5 using two different PGAM5-targeted short-hairpin RNAs (shRNAs; shRNA#1 and shRNA#2) in the two HCC cell lines (7402 and HepG2) that were more resistant to 5-fluorouracil (5-Fu) (Fig. 2b).