Although it is easy to anticipate that a possible PFKFB isoform switch during cancer transformation to PFKFB3 would give the advantage of a high glycolytic flux in malignant cells due to the enzyme’s high kinase to phosphatase ratio, nuclear PFKFB3 has been shown to drive proliferation dependent on its enzymatic function without increasing the overall cellular glycolysis22. This evidence concerns the gene PFKFB3 and cancer.