Similarly, addition of the receptor-interacting protein kinase (RIPK)1 inhibitor necrostatin-1s (Nec-1s) failed to block cell death induced by loperamide, pimozide, STF-62247 or IM/TIC, whereas Nec-1s profoundly protected HT-29 colon carcinoma cells from cell death induced by a combination of tumor necrosis factor (TNF)α, the Smac mimetic BV6 and zVAD.fmk (Fig. 3d), a well-described model of necroptosis33. The gene discussed is TNF; the disease is colon carcinoma.