To exclude the influence of a low T cell activation threshold in Med23−/− mice, we transferred purified WT or Med23−/− iNKT cells (2 × 105) into Jα18−/− mice (iNKT cell-deficient) that had been previously inoculated with B16F10 melanoma cells and injected three times with 2 μg of α-GalCer (days 0, 4, and 8). Here, MED23 is linked to melanoma.