In order to elucidate the pathway through which Enah regulates cell proliferation and metastasis in GC, we detected protein expression of several canonical signaling pathway correlated with cell proliferation and metastasis, including MAPK pathway (p-Erk1/2/Erk1/2), JAK-STAT pathway (p-STAT3/STAT3), NF-κB pathway (p-p65/p65), PI3K/AKT pathway (p-AKT/AKT) and EMT markers such as E-cadherin, Vimentin and Fibronectin in MKN45 and AGS cells. This evidence concerns the gene ENAH and gastric cancer.