In this study, we found that inhibition of MALT1 with MI-2 could suppress inflammatory molecules such as tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), IL-17α, and IL-22 in the large intestinal tissues of the in vivo mouse model of dextran sulfate sodium (DSS)-induced colitis and alleviate progression of DSS-induced colitis. The gene discussed is TNF; the disease is colitis.