Besides, YAP1 mRNA and protein expressions were greatly reduced by MLK7-AS1 knockdown in ovarian cancer cell lines (Fig. 5c and d; P < 0.01); Moreover, we further immunostained SKOV3, OVCAR3, and PEO1 cells with antibodies to YAP1, and the immunofluorescence of YAP1 was downregulated in MLK7-AS1 knockdown groups compared to that in the si-NC transfected group (Fig. 5e and f; P < 0.01). The gene discussed is MAP3K20; the disease is ovarian cancer.