(J) Representative flow cytometry scatter plot of the initial gating strategy starting from the circulating CD80 + CD86 + cells percentage (%) in the leucocyte population and (L) representative flow cytometry panels with quadrant regions of the of circulating CD80+CD86+TLR2+TLR4+cells in the CD80+CD86+cell population of HSs and SSc pts. This evidence concerns the gene CD80 and systemic sclerosis.