In the present study, we investigated the effect of ANRIL on HepG2 cells proliferation, apoptosis, migration and invasion, as well as uncovered whether ANRIL affected HCC cell biological processes through regulation of microRNA-191 (miR-191), nuclear factor kappa B (NF-κB) and Wnt/β-catenin signaling pathways. Here, CDKN2B-AS1 is linked to hepatocellular carcinoma.