Western blot analysis of the spleen, as well as ELISA for plasma protein, demonstrate reduced IL-10 protein levels in spleen and in circulating blood isolated from mice with GI dilatation, when compared to MHV-68-infected IFNγR−/− mice without GI dilatation (Figure 5B,C; p = 0.0385). This evidence concerns the gene IL10 and Vascular dilatation.