To determine whether the addition of exogenous Na-Lactate would affect the initial growth of tumors and the development of metastasis, 4T1-HRE A5NC or LDH-A KD tumor cells were mixed with Matrigel in the presence or absence of 30 mM Na-Lactate (Na-Lactate matrigel plug) [19]. Here, LDHA is linked to neoplasm.