To determine whether Tsc2-deficient cells are Vps34-dependent in vivo, we inoculated 2.5 × 106 Tsc2-deficient cells with downregulation of Vps34 (shPIK3C3) or shCTL subcutaneously into NOD/SCID/IL2gR deficient mice (n =10  mice/group) and monitored tumor growth. Here, TSC2 is linked to neoplasm.