Aiming to further investigate if TFAM/mtDNAcn is related to energetic metabolism, we performed an RNA-seq analysis in seven melanoma cell lines (WM35, WM1552C, WM1789, WM278, WM793, 1205 LU and WM1617), which we have previously evaluated for metabolic parameters, searching for gene expression alterations that could explain the correlation of mtDNAcn with glucose consumption and ATP production. The gene discussed is TFAM; the disease is melanoma.