To test the quality of RNA-seq, we screened for the expression of 5 PPAR signaling pathway related genes (FABP4, SCD, PLIN1, ADIPOQ, MSTN, the expression of PCK1 was not quantified as its expression level was too low to be detected by qPCR) using qPCR in a series of 10 pairs of muscle samples from AIS patients. The gene discussed is MSTN; the disease is androgen insensitivity syndrome.