To test the quality of RNA-seq, we screened for the expression of 5 PPAR signaling pathway related genes (FABP4, SCD, PLIN1, ADIPOQ, MSTN, the expression of PCK1 was not quantified as its expression level was too low to be detected by qPCR) using qPCR in a series of 10 pairs of muscle samples from AIS patients. This evidence concerns the gene PPARA and androgen insensitivity syndrome.