To verify the presence of the HHIP G1546A mutation, we performed pyrosequencing representing the mutant nucleotide “A” significantly on pyrograms using genomic DNA samples derived from MLN (PLM01-3 and PLM01-4) but not from normal and primary tumor tissues showing non-specific “A” peak below 5 percent (PLM01-1 and PLM01-2) (Figure S2). This evidence concerns the gene HHIP and neoplasm.