In a dose- and time-dependent manner, MTF inhibited proliferation, migration and invasion of osteosarcoma cells and this effect was associated with decreased expression of phosphorylated AKT serine/threonine kinase 1 (p-Akt) and vimentin (VIM), and increased expression of PTEN and cadherin 1 (CDH1) [70]. The gene discussed is AKT1; the disease is osteosarcoma.