To further demonstrate how EBOV interacts histologically in the GI tract we included a hematoxylin and eosin (H and E), immunohistochemistry (IHC), ISH, smooth muscle actin (SMA)/EBOV glycoprotein (GP) immunofluorescence assay (IFA), CD31/EBOV glycoprotein (GP) IFA, and CD68/EBOV GP IFA staining of one of the three NHPs that demonstrated gram-negative bacterial infection at necropsy (Figure 6). Here, ART4 is linked to gram-negative bacterial infections.