CSBG-induced mechanical allodynia in mice deficient in inflammasome component genes (Figure 3B), histamine receptor H1 (Hrh1)-deficient mice (Figure 3C), non-obese diabetic/severe combined immunodeficiency mice (lacking T and B cells, Figure 3C), clodronate liposome-treated mice (lacking macrophages, Figures 3D and 3E), Ly6G antibody-injected mice (lacking neutrophils, Figures 3F and 3G), ibuprofen-treated mice (Figure 3H), and TNF-α-antibody-injected mice (Figure 3I) was indistinguishable from that in wild-type mice. This evidence concerns the gene TNF and severe combined immunodeficiency.