Recombination analysis of expressed VH−C, Vκ-C and Vλ-C segments, respectively, revealed that a variety of rearranged Ig heavy chains (IgG1, IgG3, IgM) and also Igκ and Igλ light chains were detectable in the melanoma-derived TAM (Fig 4B). This evidence concerns the gene CD40LG and melanoma.