Limitations of our study includes the relative disadvantages of using mRNA from FFPE samples due to RNA degradation and fragmentation during the fixation process and the missing information of the spatial distribution of immune cells within the tumor in comparison with the IHC method; with the latter limitation in mind, we are in the process of evaluating CD3, CD8, and FOXP3 protein expression in the same patient cohort, in order to verify whether the qRT‐PCR findings correlate to the IHC results. Here, FOXP3 is linked to neoplasm.