CS and osteosarcoma: To determine if one specific type of cell surface HSPG (CS-HSPG) could be responsible for the negative action of HS on ATXβ cell binding, we first screened by real-time PCR the expression levels of mRNAs for all 10 CS-HSPG (4 SDC; 6 GPC) in human osteosarcoma, prostate and breast cancer cell lines.