It is interesting that SPOP knockdown caused increase of CYCLIN E1 only in four cell lines, DU145 and PC3, two prostate cancer cell lines, SV-HUC-1 and T24, two bladder cell lines, but not in others, including four kidney cell lines (ACHN, 786-O, 769-P, and HEK293), one colon cancer cell line (HCT116), two liver cell lines (HepG2 and HL7702), one bone osteosarcoma cell line (U2OS), one cervical cancer cell line (HeLa), one bladder cancer cell lines (UM-UC-3) and one prostate cancer cell line (22RV1) (Fig. 2a, b and Sup. The gene discussed is SPOP; the disease is bone osteosarcoma.