Decreased Y397-FAK autophosphorylation following 0.1 μM BI 853520 treatment occurred within 10 min and was substantially reduced at least for the following 48 h (Fig. 1c), demonstrating a fast and potent inhibition of FAK by BI 853520 in this highly metastatic murine breast cancer cell line. The gene discussed is PTK2; the disease is breast carcinoma.