Furthermore, Bio-Plex immunoassays showed that the secreted CCL2 protein was the most significantly increased cytokine in the LNMAT1-transduced bladder cancer cells, but the secreted CCL2 protein levels were decreased in the LNMAT1-silenced cells (Fig. 4c, d), results that were further validated by qRT-PCR and ELISA (Fig. 4e–h). This evidence concerns the gene DUXAP9 and urinary bladder carcinoma.