Cell viability, colony formation, wound closure, and matrigel invasion assays showed that knockdown of FIS1 by RNA interference in SMMC7721 remarkably inhibited both cell growth (Fig. S4B and S4C) and metastasis (Fig. S4D and S4E) abilities of HCC cells, suggesting that MTP18 may exert its oncogenic function by regulating the mitochondrial morphology. This evidence concerns the gene MTFP1 and hepatocellular carcinoma.